Leica DMLS Bedienungsanleitung Seite 40

Using an Allen key (1.1) adjust the horizontal
position (29.3) of the lamp holder until the
slightly brighter stripe in the reflection of the
lamp filament is in the centre of the brighter
area (Fig. 30), as marked by the dot you made.
Then move the reflection of the lamp filament
with the vertical adjustment (29.2) to the centre
of the range of movement.
Lamphousing 106 z with halogen lamp and gas
discharge lamps (Fig. 31 and 32).
The image of the light source is focused with the
collector (31.6).
The adjustment principle is similar for all light
sources:
Move the reflection of the lamp filament or
discharge arc to the side (32a) by turning the
adjustment screws on the back of the
lamphousing (31.2 and 31.4). Focus the direct
image of the filament or discharge arc (31.6) and
adjust as follows (32b, 31.5 and 34.6):
Halogen lamp: just above or below the center
marking you made (Fig. 32b).
First focus the reflection (31.3) and then move it
symmetrical to the direct image (31.2 and 31.4)
inside the brighter circle (32c).
Mercury (Hg) and xenon (Xe) lamps
Move the direct image (32a, b) to the centre of
the brighter circle with the horizontal and
vertical (31.2 and 31.4) adjustment of the holder.
Focus the reflection (3.3) and adjust the mirror
(3.2 and 3.4) until the reflection coincides with
the direct image (32c).
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Attention:
Caution with Hg and Xe lamp s:
Be careful not to project the reflection on the
electrodes for long, as there is a risk of
explosion if they overheat. The two elec-
trodes can just be seen in the extension of
the symmetry plane of the discharge arc.
Replace spent burners in good time and
dispose of in an environment-friendly way.
Do not open the lamphousing until the lamp
has cooled down and you have disconnected
it from the mains. Wear protective clothing
(gloves and mask) when using Xe lamps. Hg
lamps take a few minutes to reach their full
intensity; they do not ignite when hot.
Filter cube, objective, tube factor
Disengage the adjustment lens. Focus the
specimen in transmitted light first if possible.
Select a filter cube to suit the excitation and
emission spectrum of the specimen and switch
it into the light path (31.10), → p. 15 for assembly.
Use high-aperture objectives (immersion) to
obtain optimum image intensity; open the iris
diaphragm in the objective if applicable (Fig. 21).
Trinocular tube* with switchable beamsplitter:
switch to highest possible intensity for visual
observation (37.4). Switch magnification chan-
ger* (36.1), if present, to factor 1x. Protect the
immersion oil from impurities to avoid disturbing
fluorescence. Use low-fluorescence mounting
media, coverglasses and specimen slides!