Measurements With The Microscope; Linear Measurements - Leica DM1000 Bedienungsanleitung

10. Measurements with the Microscope

10.1 Linear Measurements

The following are
measurements:
- Graticule with scale division in eyepiece or
HC FSA 25 PE tube with diapositive overlay
device or a digital linear measuring eyepiece
- Stage micrometer for calibration.
(→ Fig. 50, p. 48)
Micrometer Value
The micrometer value of the objective-eyepiece
combination used must be known before the
measurement, i.e. the distance in the specimen
that corresponds to the length of a division on
the graticule used.
Calibration:
• Align the stage micrometer and the graticule
parallel to each other by rotating the eyepiece
and adjust the zero marks of both scales to
exactly the same height.
• Read how many scale divisions of the stage
micrometer correpond to how many on the
microscope scale (graticule).
• Divide the two values. The result is the
micrometer value for the total magnification
that has just been used.
Example:
If 1.220 mm of the stage micrometer corresponds to 50
divisions of the measurement scale, the micrometer
value is 1.220:50 = 0.0244 mm = 24.4 µm. For extremely
low objective magnifications it may be that only part of
the measurement scale can be used for calibration.
required for linear
10. Measurements with the Microscope
Notes:
If using a magnification changer:
Remember to take the additional magnification
value into consideration!
recommend you calibrate each objective
separately instead
micrometer values of the other objectives from
the calibration of one objective.
Measurement errors may occur if the eyepiece
is not pushed into the tube as far as the stop.
Particularly large object structures can also be
measured on the stage with the verniers (0.1 mm);
the distance to be measured could be calculated
from a combined x and y measurement.
We strongly
of extrapolating the
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