• Remove the probe from environments that will damage the epoxy materials used in the probe tip
(e.g., keep the probe away from acetone, toluene, methylene chloride, xylene and other strong
organic solvents).
• Do not use the probe in temperatures more than 60 ºC (140 ºF). Thermal cycling can decrease the
life of the probe.
• For semi-solids use, carefully twist the probe to make sufficient contact with the sample to the
sensor.
• When semi-solids are tested, make sure that solid objects (i.e., bone or gristle) do not scratch the
sensor.
• Cool samples to room temperature to maximize probe life.
• Always use new buffers and new rinse solutions.
Prepare the probe for storage
Do not use the probe for long-term pH measurement applications.
Note: No electrode storage solution is necessary.
A new probe usually has visible reference gel at the tip of the probe, as well as in the interior of the
rubber dust cap. To remove the gel, carefully clean with a soft-bristled toothbrush and mild soapy
water (a few drops of dish soap in a warm cup of water). The gel can continue to show for two to five
days. Do not put the rubber dust cap on the probe until all of the reference gel is removed. Do not
complete the steps that follow until all reference gel is removed from the probe and the interior and
exterior of the rubber dust cap.
Prepare the probe for storage
1. Put the new probe (or the probe that was in extended storage) in pH 7 buffer for at least
5 minutes.
2. Stir the probe in pH 7 buffer solution to dislodge air bubbles.
3. Use fresh deionized water to rinse the probe.
4. Dry the probe with a lint-free cloth.
5. Put the rubber dust cap back on.
6. Keep the probe in dry storage when not in use.
Calibrate the ISFET probe
Refer to
Prepare the probe for storage
Do not use the probe for applications that cycle between hot and room temperature samples.
Note: Remove other active or non-active measurement devices during pH or conductivity measurement. Other
devices, even AC power, can cause interference.
Note: Only do calibrations away from the sun. Direct sunlight can cause unstable readings or difficulty in
calibration.
1. Connect the probe to the meter.
2. Set the meter power to on.
3. Clean the probe with new deionized water and dry with a lint-free cloth.
4. Put the probe in the pH 7 buffer.
5. Clean the probe with deionized water and dry with a lint-free cloth.
6. Put the probe in the second buffer (pH 4 or pH 10).
7. Read the results.
8. If the result is not correct, the probe is not correctly hydrated.
Soak the probe for another 5 minutes in pH 7 buffer, then do the calibration again.
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on page 16 before calibration.
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