Aspirating And Dispensing Liquids; Aspirating Liquids; Dispensing Liquids - VWR Ultra High-Performance VE2 Handbuch

• Operate the pipettor pushbutton slowly and smoothly
when liquid aspiring and dispensing.
• The depth of tip immersion in the aspired liquid should
be kept at necessary minimum, and should remain
constant during aspiration (Table 2).
• While operating, the pipettor should be held in a vertical
position.
• Each new tip should be pre-rinsed.
• The tip should be replaced with a new one if:
- a different type of liquid is handled;
- a different liquid volume is dispensed;
- visible droplets of liquid remain in it.
• Pipetted liquid should not enter the pipettor shaft. To
ensure this:
- press and release the pipetting pushbutton slowly
and smoothly;
- do not put the pipettor away if there is any liquid in
the tip;
- do not invert the pipettor;
- do not set and aspire volumes exceeding nominal
values.
• Before aspiring liquid of temperature that differs from
ambient temperature, it is recommended to rinse the
tip several times with the aspired liquid.
CAUTION: After pipetting acids and aggressive solu-
tions, it is recommended to unscrew the pipettor and
rinse the pipettor plunger, seal and shaft with distilled
water.
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4. ASPIRATING AND DISPENSING LIQUIDS

4.1 ASPIRATING LIQUIDS

A B C
Fig. 4
• The pipetting pushbutton (fig. 1A) should be pressed to
the first stop (fig. 4A),
• While holding the pipettor in a vertical position, immerse
the tip into the aspired liquid. Depth of the tip immer-
sion depends on the pipettor model. The recommend-
ed values are shown in Table 2. The tip may aspire air if
immersed to a depth lower than recommended or if the
pipetting pushbutton is released quickly,
• To aspire liquid release the pipetting pushbutton
smoothly (fig. 4B),
• Wait for about 1 second before withdrawing the tip
from the aspired sample, fig. 4.
WARNING! Do not touch the used tip.
Volume 2,10 μl
Depth <1mm
Table 2

4.2 DISPENSING LIQUIDS

• While holding the pipettor slightly inclined from 10 to
40° off the vertical, place the tip orifice against the
inside wall of the receiving vessel.
• Then, press the pipetting pushbutton smoothly to the
first stop, dispensing the liquid (fig. 4C).
ENGLISH
D E F
20, 50, 100 μl 200, 1000 μl
2-3mm 2-4mm
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