Gel Preparation; Gel Pouring - VWR shiroGEL Horizontal 96 Bedienungsanleitung

Gel Preparation:

1. For a standard 0.7% agarose gel, add 0.7 grams of agarose to 100 ml of 1x TAE
or TBE solution. The same 1 x solution should be used in the tank buffer solution.
For a 5mm thick gel 60ml should be used.
2. Add the agarose powder to a conical flask.
3. Add the appropriate amount of 1x TAE or TBE solution from the table above. To
prevent evaporation during the dissolving steps below, the conical flask should be
covered with parafilm.
4. Dissolve the agarose powder by heating the agarose either on a magnetic hot
plate with stirring bar or in a microwave oven. If using the microwave method, the
microwave should be set at around a 400 watt or medium setting and the flask
swirled every minute. The solution should be heated until all crystals are dissolved.
This is best viewed against a light background. Crystals appear as translucent
crystals. These will interfere with sample migration if not completely dissolved.
The gel must be cooled to between 50°C and 60°C degrees

Gel Pouring:

Casting Dams
Traditional Tape
Using trays with Casting Dams:
1. Fit the casting dams over each end of the tray and place onto a level surface.
The dams should be fitted so that there is no gap between the sides of the
tray and the groove in the dams. This will ensure that there is no possibility of
gel leakage.
2. Place the comb(s) in the grooves. Each tray has more than one comb grove
so that multiple combs can be used. Using multiple combs increases sample
number available per gel but decreases run length and care must be taken to
before pouring.
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