Solutions - VWR shiroGEL Horizontal 96 Bedienungsanleitung

Solutions:

1x TAE 40 mM tris (pH 7.6), 20 mM acetic acid, 1 mM EDTA.
50x (1L) dissolve in 750 ml distilled water:
242 g tris base (FW = 121)
57.1 ml glacial acetic acid
100 ml 0.5 M EDTA (pH 8.0).
Fill to 1 litre with distilled water.
1x TBE 89 mM tris (pH 7.6), 89 mM boric acid, 2 mM EDTA
10x (1L) dissolve in 750 ml distilled water:
108 g tris base (FW = 121)
55 g boric acid (FW = 61.8)
40 ml 0.5 M EDTA (pH 8.0)
Fill to 1 litre with distilled water.
Sample Loading Dye
10x sample buffer stock consists of 50% glycerol, 0.25% bromophenol blue, and
0.25% xylene cyanole FF in 1x TAE buffer. Only 1–10 ml of the 10x loading dye
should be prepared.
Ethidium Bromide Solution
Add 10 mg of Ethidium Bromide to 1 ml distilled water.
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