StarLab ErgoOne Bedienungsanleitung Seite 9

Reverse pipetting
When pipetting critical viscous media,
the precision and accuracy can be
increased through 'reverse pipetting'.
In reverse pipetting, a larger volume
is aspirated than that indicated on the
volume display. As a result, a small
amount of residual liquid remains in the
pipette tip after the pipetting process.
How to reverse pipette:
– Secure the pipette tip.
– Press the volume control button
until it reaches the second stop
position.
– Hold the pipette upright and
immerse the pipette tip approx.
3mm into the liquid.
– Allow the volume control button
to return slowly back to the starting
position.
– Hold the pipette tip in the liquid for
a further 2 seconds to avoid sucking
in air.
Note: Pre-wetting
Some liquids (eg. solutions containing protein, organic solvents, or viscous liquids)
leave a film on the inside of the pipette tip. To ensure maximum precision and
accuracy, STARLAB recommends pre-wetting each new tip first by aspirating and
dispensing the liquid two or three times. Alternatively, TipOne® RPT pipette tips are
special, surface-treated tips that ensure improved flow behaviour for 'tricky' liquids.
Ask your STARLAB representative for more information.
6
· ErgoOne · User Manual
– Remove the pipette tip from the
liquid.
– Place the pipette tip against the
inner wall of the tube or vessel, at an
angle of 30 – 45°.
– Press the volume control button
slowly and evenly until it reaches the
first stop position (do not use the
blow-out function).
– Keeping the volume control button
pressed, remove the pipette tip from
1
the tube or vessel before blowing
out the residual liquid into a
separate container.
– Allow the volume control button
to return slowly back to the starting
position.
1
– Press the tip ejector button
eject the pipette tip.
1
1
1
to
2